Based on this criterion, we predicted that conserved peptide C6_30 would be able to identify infection caused by four of the six T. cruzi DTUs (Figure 4A), whereas peptides A6_30_col and B2_30_y are expected to identify infections caused only by TcI and TcVI (Figure 4B) and TcII and TcVI (Figure 4C), respectively. This evidence concerns the gene TCN2 and infection.