Results from our Western blotting showed that LC3 shRNA plasmid transfection and GST treatment very efficiently inhibited rapamycin-induced autophagy with down regulation of the autophagic signaling molecules LC3 and Beclin 1 and also modulated expression of the apoptosis regulatory proteins Bax (pro-apoptotic) and Bcl-2 (anti-apoptotic) essentially to increase the Bax:Bcl-2 ratio to trigger the mitochondrial pathway of apoptosis in human malignant neuroblastoma cells not only in culture models but also in animal models. The gene discussed is BCL2; the disease is neuroblastoma.