To elucidate whether Dectin-2 deficiency affected the host protection caused by PPV vaccination against pneumococcal infection, spleen cells were prepared from WT and Dectin-2KO mice on day 14 after vaccination and transferred into uninfected WT mice that were then infected with S. pneumoniae 24 h after the cell transfer (see Figure S2A). The gene discussed is CLEC6A; the disease is pneumococcal infection.