Preliminary morphological studies of crypts isolated from APCmin/+ and Tg(A33-CreERT2);Apcfl/fl mice (24 hour Tamoxifen induction to induce truncation of APC) indicate no occurrence of multiple budding on a single crypt (not a surprising result considering no adenoma were present in the colons of these mice) (Figure S6). The gene discussed is APC; the disease is adenoma.