RSPO1 stimulation of LGR4 potentiates Wnt signaling which is generally oncogenic [13-15,37], and overexpression of RSPO2 and RSPO3 is associated with carcinogenesis in human colon cancer as well as in mouse models of breast and colon cancer [20,38-40]. This evidence concerns the gene LGR4 and malignant colon neoplasm.