Sixteen proteins identified as differentially expressed using mean expression values of all samples per tumor (AKT, pAKT, Angiopoietin 2, B-Raf, p1068EGFR, p1148EGFR, FAK, pGSK-3β, pGSK-3β, Hif-1α, JNK/SAPK, mTOR, pmTOR, p38 MAPK, pPRAS40, PRAS40, PTEN, pPDGFR, S6RP) were not detected when analyzing randomly selected single samples per case. The gene discussed is AKT1; the disease is neoplasm.