CCNE1 and esophageal cancer: To investigate whether miR-139-5p affects cell cycle and invasiveness through downstream genes regulated by NR5A2 in esophageal cancer, the mRNA levels of cyclin D1 and cyclin E1 were determined during cell cycle arrest, and the mRNA of MMP9 was detected during cell invasiveness in EC109 cells after transfection with miR-139-5p mimic using qRT-PCR.