C6 glioma cells were also considered a viable model of glial cells during acidosis and postischemic brain edema, where pHi served as a secondary readout for NHE involvement in osmotic swelling and regulatory volume increase (Jakubovicz et al., 1987; Kempski et al., 1988; Jakubovicz and Klip, 1989; Staub et al., 1994) under mildly acidotic conditions (pHe 6.0–7.0). This evidence concerns the gene SLC9C1 and glioma.