To regulate epithelial- or mesenchymal-like phenotypes, we infected two mesenchymal-like liver cancer cell lines, MM189 and BL322, with a retroviral vector encoding mouse Klf4 (MM189 PB-Klf4 and BL322 PB-Klf4) or empty vector (MM189 PB and BL322 PB) and determined ectopic Klf4 expression with immunoblot assay (Figure 1a). This evidence concerns the gene KLF4 and liver cancer.