This is in line with the literature; Weigelt et al. have shown that AU565 breast cancer cells grown in Matrigel cultures are more sensitive to the HER2 inhibitory antibody trastuzumab but less sensitive to HER2 dimerization inhibiting antibody pertuzumab when compared to the same cells grown in 2D cultures [10]. This evidence concerns the gene ERBB2 and breast carcinoma.