mRNA isolated from CD133+ cells derived from the melanoma cell line LM-MEL-34 was used to amplify the 5′ end of the PROM1 transcript with the help of a pair of forward primers targeted to a RACE adapter and the other pair of reverse primers specifically targeted to 5′ exon of PROM1 (Table 2, Figure 3A). Here, PROM1 is linked to melanoma.