First, we expanded initial literature-based regulatory network of hESC using a set of seven perturbation experiments including—NANOG, SOX2, and OCT3/4 RNAi knockdowns in human embryonal carcinoma cells (NCCIT) (Greber et al., 2007b); bFGF, BMP4 and ACTA protein stimulation in H1 embryonic stem cells (Greber et al., 2008); GADD45G over-expression in NCCIT cells (Jung et al., 2010) from our previously published Embryonic Stem Cell Database (ESCDb, Figure 2B) (Jung et al., 2010). This evidence concerns the gene NANOG and embryonal carcinoma.