First, we expanded initial literature-based regulatory network of hESC using a set of seven perturbation experiments including—NANOG, SOX2, and OCT3/4 RNAi knockdowns in human embryonal carcinoma cells (NCCIT) (Greber et al., 2007b); bFGF, BMP4 and ACTA protein stimulation in H1 embryonic stem cells (Greber et al., 2008); GADD45G over-expression in NCCIT cells (Jung et al., 2010) from our previously published Embryonic Stem Cell Database (ESCDb, Figure 2B) (Jung et al., 2010). Here, ACTA1 is linked to embryonal carcinoma.