INS and type 2 diabetes mellitus: Using real-time RT-PCR, it was shown that the expression of the type 3 AC (AC3) in the striatum and hypothalamus of Goto-Kakizaki rats, a spontaneous animal model of T2DM, was higher compared with Wistar rats, and the 15-day treatment of Goto-Kakizaki rats with insulin led to a decrease of the enzyme expression [24].