Further subgroup analysis based on CRC patients’ treatment showed that elevated S100A4 levels were markedly related with worse OS in CRC patients treated by surgery (pooled HR = 2.27, 95% CI: 1.74–2.96, P <0.001), without any evidence of heterogeneity (P = 0.68, I2 = 0.0%). Here, S100A4 is linked to colorectal carcinoma.