To confirm the participation of the actin cytoskeleton and its rearrangement during E. invadens encystment, Cytochalasin D (CD), an actin filaments polymerization and elongation inhibitor [23], and Y27632, a drug capable of selectively inhibiting RhoA kinase (ROCK-2), thereby suppressing induced RhoA activity [24], were used to analyze encystment efficiency (Figure 7(a)), actin polymerization (Figures 7(b) and 7(c)), and cyst wall formation (Figure 7(d)). Here, ROCK2 is linked to cyst.