We found that the CpG islands in the miR-375 promoter region were hypermethylated (76–96%) in AR-negative PCa cells (PC3 and DU145), but only very limited methylation (0.0–4.4%) was seen in AR-positive PCa cells (LNCaP, C4-2, 22Rv1) (p<0.001, Fig. 2B). This evidence concerns the gene AR and posterior cortical atrophy.