In this study, investigations were carried out to determine how crown daisy root exudate disrupted M. incognita chemotaxis and decreased nematode infection in controlled and soil conditions, to identify and quantify compounds exuded from crown daisy roots, to determine the function of the Mi-flp-18 gene in chemotaxis and infection, and to confirm the compounds that influenced nematode chemotaxis and interfered with Mi-flp-18 expression to disrupt nematode infection. Here, MITF is linked to Nematoda infectious disease.