The pathways involved in the effects of ROCK knockdown in glioblastoma cells were analyzed using Western blot analysis to determine the protein expression levels of phosphoLimK, phosphoRac1/cdc42, cyclin D1, Akt1, phosphoAkt, β1-integrin, β-catenin, phosphoERK1/2, and RhoA in cells with a stable ROCK1 and ROCK2 knockdown (Fig. 7a, b) as well as in glioma cells treated with 100 μM of Rho kinase inhibitor (Fig. 7c). This evidence concerns the gene ROCK2 and central nervous system cancer.