CCND1 and central nervous system cancer: The pathways involved in the effects of ROCK knockdown in glioblastoma cells were analyzed using Western blot analysis to determine the protein expression levels of phosphoLimK, phosphoRac1/cdc42, cyclin D1, Akt1, phosphoAkt, β1-integrin, β-catenin, phosphoERK1/2, and RhoA in cells with a stable ROCK1 and ROCK2 knockdown (Fig. 7a, b) as well as in glioma cells treated with 100 μM of Rho kinase inhibitor (Fig. 7c).