To establish methods to further verify the induction of malaria vaccine antigen-specific human CD8+ T cells and to determine the total magnitude of this CD8+ T cell response, a series of tetramers were prepared (Table 1), each consisting of one of three HLA-A* or one HLA-B* class I molecules and each presenting one of six distinct AMA-1 epitopes. The gene discussed is CD8A; the disease is malaria.