Consistent with the clamp data, phosphorylation of the serine/threonine kinase, AKT, is increased in livers isolated from insulin-stimulated KLF15-/- versus WT mice after 8 weeks of HFD; however, these mice also exhibit increased hepatic expression and phosphorylation of UPR genes compared to WT littermates, providing evidence for uncoupling of ER stress and insulin resistance (Figure 2A). This evidence concerns the gene KLF15 and Insulin resistance.