To further examine the relationship between MAP4K4 and Pyk2 stimulated glioma cell migration, control SF767 glioma cells or SF767 glioma cells stably transduced with a shRNA targeting Pyk2 were transfected with MAP4K4, and the effect on cell migration was assayed with a radial migration assay (Figure 6). This evidence concerns the gene PTK2B and glioma.