APOA1 and limb ischemia: Three groups were constituted: Control (MI, myocardial I/R alone), RIPC+MI (10-min limb ischemia followed by 10-min reperfusion initiated 20 minutes prior to coronary artery occlusion), and ApoA-I+MI (ApoA-I injection 10 minutes prior to coronary artery occlusion), as illustrated in Figure 4A.