As shown in Table 2, ESCC samples with high-expression of USP9X had significantly higher frequencies of T3–T4 cases compared with the low-expression group (51.1% vs. 31.6%, respectively; P = 0.046) and high expression of USP9X was more prevalent in node-positive than in node-negative cases (51.0% vs. 30.2%, respectively; P = 0.032). Here, USP9X is linked to esophageal squamous cell carcinoma.