MSLN and gastric cancer: In 5 of the 6 investigated gastric cancer cell lines (MKN-1, MKN-7, MKN-74, NUGC-4 and TMK-1), ERC/mesothelin mRNA and C-ERC protein were detected by RT-PCR (Fig. 1), flow cytometry (Fig. 2A) and immunohistochemistry (Fig. 2B).