As opposed to p107+/−/p130−/−/RbLox/Lox/α-MHC-Cre+/− (Hetp107/KOp130/KOαMHC-Rb, H/K/K) and p107−/−/p130+/−/RbLox/Lox/α-MHC-Cre+/− (KOp107/Hetp130/KOαMHC-Rb, K/H/K) mice, which survive to adulthood, 3KO pups were stillborn or died at E14.5–E15.5 from heart failure as manifested by large pericardial effusions and anasarca. This evidence concerns the gene RB1 and pericardial effusion.