However, GRP at doses of 5 and 50 pmol produced quite similar significant and sustained increases in PDA (maximum increase; 50 pmol: +30%; Fig. 2D) but the increase in ƒH did not reach the level of statistical significance in the two-way ANOVA test (maximum increase 50 pmol: +20%; Fig. 2E). The gene discussed is GRP; the disease is Patent ductus arteriosus.