To determine the TIMP-1 expression levels in human prostate cancer cells, we assembled a panel of human prostate cancer cell lines (LNCaP, VCaP, LAPC-4, 22RV1, DU145, PC3 and PC3/M), an immortalized normal prostate epithelial line (RWPE-1) and its malignant derivative (WPE1-NB26-65) [39]. Here, TIMP1 is linked to prostate cancer.