Furthermore, quantification of the expression of endothelial cell markers by qRT-PCR in subcutaneous tumours showed a slight decrease in the expression of VE-cadherin (Cdh5) in MB49-I-shDcn1 and MB49-I-shDcn2 tumours, and of VEGFR-2 (Kdr) and CD31 (Pecam1) in MB49-I-shDcn1, as compared to MB49-I-Scr tumours (Fig 5G). This evidence concerns the gene PECAM1 and neoplasm.