ORC1 and Mungan syndrome: Consistent with this interpretation, cells derived from MGS patients with mutations in Orc1 show reduced recruitment of ORC subunits and replicative helicase components to chromatin, and are compromised in their ability to replicate plasmid DNA (Bicknell et al., 2011b); recent work has also shown a link between origin licensing factors and non-replicative cellular processes, such as centrosome duplication and cilia formation, in MGS patient cells and in cells depleted of licensing proteins (Hossain and Stillman, 2012; Stiff et al., 2013).