To evaluate the levels of COX-2 and PPAR-γ expression in nine CRC cell lines, namely SW116, SW480, SW620, C205, T84, HT29, HCT116, CACO-2 and DAuD1, representing various grades of malignancy, the total protein extracted from these lines was evaluated by western blotting using monoclonal anti-COX-2 and -PPAR-γ antibodies (Fig. 6). This evidence concerns the gene PTGS2 and colorectal carcinoma.