NMNAT3 and glaucoma: This increase in autophagic flux represents autophagy induction rather than a block in downstream steps, such as autophagolysosomal maturation.41 It was shown that the activation of autophagy increased protein levels of LC3-II and Beclin1 and decreased p62 in neuroblastoma SH-SY5Y cells.42 In addition, we observed that Nmnat3 transfection decreased p62 protein levels and increased LC3-II protein levels in the optic nerve both with and without glaucoma.