Furthermore, we determined ERK, p-ERK, AKT, p-AKT, and PTEN expression in melanoma cells newly isolated from brain and extracerebral distant metastases of melanoma patients (TüMel32H, TüMel32; TüMel19H, TüMel21H, TüMel25H; TüMel22, TüMel23, TüMel27, TüMel28, TüMel30). The gene discussed is PTEN; the disease is melanoma.