To determine whether this antibody was still reliable for probing hCtr1 protein expression, we performed Western blotting using whole cell extracts prepared from SCLC cells and cisplatin-treated SCLC cells, and SCLC cells transfected with a dominant-negative mutant CTR1 complementary DNA (a positive control for hCtr1 expression). The gene discussed is SLC31A1; the disease is small cell lung carcinoma.