In this study, we simultaneously confirmed that the anti-obesity abilities of DOW-RMD in inhibiting PPARγ and C/EBPα expression in differentiation and lipoprotein lipase activity in lipogenesis were contributed to by the DOW-increased monascin and ankaflavin levels and the ions of DOW, respectively. This evidence concerns the gene LPL and obesity due to melanocortin 4 receptor deficiency.