The results of this study demonstrated different spectra of viral strains of HCV E1-E2 isolates showing synonyms and non-synonyms substitutions, finding glycosylation sites and different epitope domains that could be used to produce monoclonal or polyclonal antibodies targeting both linear and conformational epitopes of envelope glycoprotein E2 that have been shown to inhibit cellular binding of HCV-LP (HCV- Leucoplasts), entry of HCVpp (HCV pseudo-particles) and infection of HCVcc (HCV cell culture). This evidence concerns the gene ERVW-1 and infection.