IL10 and systemic lupus erythematosus: We hypothesized the presence of transcription factors activated in SLE patients upregulating IL10 expression and prepared nuclear extracts of peripheral blood lymphocytes from active SLE patients (defined as SLEDAI score≥4) [20], [21] to perform electrophoretic mobility shift assays (EMSA) for testing allelic differences in transcription factor binding conferred by the SLE-associated SNPs rs3122605, rs3024505, rs3024495 and rs3024493.