This was corroborated by increased IFN-γ and TNF-α released into the supernatant culture medium from NK cells in contact with GBM cells compared to supernatants from tumor cells only, (IFN-γ: One-Way ANOVA6.54, p=0.03; n=11; and TNFα: One-Way ANOVA 9.59, p=0.0083; n=11). This evidence concerns the gene TNF and glioblastoma.