In order to assess whether decreased H2A.Z levels were due to mTOR pathway inhibition by sirtuin 1, the selected PCa cell lines were stably silenced for FRAP, the gene encoding mTOR protein, and silencing validation was performed by qRT-PCR (Supplementary Fig. 1B) and Western blot (Fig. 2 B1 and 2 B2). This evidence concerns the gene MTOR and posterior cortical atrophy.