When compared for their cytotoxic activity against tumor targets endogenously expressing brachyury (Fig. 2B), CD8+ T cells purified from the PBMCs of donors 3 and 4 were able to efficiently lyse SW480 cells (HLA-A2+ and brachyury-high), while lower lysis was observed against the MCF7 breast carcinoma cell line that is HLA-A2+ but expresses lower levels of the target brachyury (Fig. 2C). This evidence concerns the gene CD8A and breast carcinoma.