In subsequent studies employing RT-PCR [5, 11, 21] or monoclonal antibodies in western blot or immunohistochemistry [6], the expression of brachyury was also demonstrated in a range of human carcinomas, including breast, lung, colorectal, pancreatic, and ovarian, and not in the majority of normal tissues evaluated. The gene discussed is TBX1; the disease is carcinoma.