Figure 3C shows a mouse injected with PC3-Epi cells, where a tumor in the left femur was predominantly mesenchymal type, while the tumor in the right displayed a highly epithelial phenotype. Importantly, we found similar mesenchymal-epithelial cell plasticity in a metastatic tumor isolated from the femur of a patient with advanced prostate cancer. IHC staining of tumor sections with E-cad and ZEB1 antibodies showed regions with both highly epithelial (high E-cad, low ZEB1) and with mesenchymal characteristics (low E-cad and high ZEB1) (Figure 3D). This evidence concerns the gene ZEB1 and metastatic neoplasm.