The results from Western blots also confirmed the decreased phosphorylation of Akt, mTOR, P70S6k, S6, and 4E-BP1 but increase in cleaved caspase-3 and cleaved PARP in rapamycin-treated mice tumor (Fig. 6 C), The suppressed expression of Ki67 in rapamycin-treated mice displayed the inhibitory effects of rapamycin in tumor cell proliferation (Figs. 6 A and B, P<0.05), while increased apoptotic cells by TUNEL staining and cleaved caspase-3 (Fig. 6 E) as well as cleaved PARP (Fig. 6 F).for indicated the effects of rapamycin apoptosis on the tumor cells (P<0.05). This evidence concerns the gene MKI67 and neoplasm.