To explore the effect of cyclin E2 on pancreatic cancer cell growth, cyclin E2 siRNA was transfected into each of the three PDAC cell lines, and the knockdown was confirmed by qRT-PCR analysis, which showed that the expression of cyclin E2 in the siRNA-transfected group was significantly decreased compared with the control cells (0.201-fold in Capan-2, 0.274-fold in SW-1990, and 0.327-fold in Panc-1) (Figs. 3D–3F). This evidence concerns the gene CCNE2 and familial pancreatic carcinoma.