To determine whether PKN1 and PKN2 inhibit Wnt/β-catenin-dependent gene expression in several established melanoma cell lines, we transfected siRNAs targeting PKN1 and PKN2 into A2058, SKMEL5, COLO-829, and Mel-624 (Fig. 3, right panel) cells. The gene discussed is PKN1; the disease is melanoma.