We observed that a commercial antibody that recognizes an activation loop phosphorylation of PKN1 (pPKN; Thr-774, a site distinct from that dephosphorylated following stimulation with WNT3A ligand) detects multiple bands in immunoblots of A375 (Fig. 2B, right blot) and A2058 (Fig. 2C, lane 8, see arrows) melanoma cell lines. The gene discussed is PKN1; the disease is melanoma.