We further found that the percentage of melanoma cells expressing AnnexinV following stimulation with WNT3A and BRAFi was significantly increased in cells transfected with siRNAs targeting PKN1 as compared with those transfected with negative control siRNA (A375 representative plots in Fig. 5B and quantification in Fig. 5C, p < 0.01; Mel-624 quantification in Fig. 5D, p < 0.001). This evidence concerns the gene ANXA5 and melanoma.