Annexin-V/PI double staining showed that treatment with C2 for 24 hrs induced eminent apoptosis of HNSCC cells, as Figure 2A shows that the apoptotic rate was 58.1% in Cal27 treated with 0.8 μM C2, compared with 11.5% in cells treated with DMSO. The gene discussed is ANXA5; the disease is head and neck squamous cell carcinoma.