The most significant associated diseases and disorders associated with the four biomarkers were inflammatory response (CEA, MIF, OPN/SPP1), cancer (all four), gastrointestinal disease (all four), cardiovascular disease (MIF, OPN/SPP1, TFRC) and infectious disease (MIF, OPN/SPP1, TFRC), supporting that these biomarkers have a biological role in CRC. This evidence concerns the gene SPP1 and cardiovascular disorder.