At the same time, apoptosis (assessed by immunohistochemical detection of cleaved caspase 3) was not induced to a significant extent by LY294002 (data not shown).To further examine a possible association of AKT activation levels and DcR3 expression, we subjected nine pairs of freshly obtained human RCC tissue and adjacent normal renal tissue to immunoblot analysis. Here, CASP3 is linked to renal cell carcinoma.