To investigate the effect of unglycosylated and anchorless PrP on human PrPSc formation, we performed the PMCA assay in which human PrPSc from brain homogenates of an iatrogenic CJD (iCJD) was used as the seed while human PrPC from brain homogenates of transgenic mice expressing wild-type human PrP-129V was used as the substrate. Here, PRNP is linked to Creutzfeldt Jacob disease.