Notably, TNPO3, NUP358, and NUP153 are not absolutely required for HIV-1 infection of transformed cell lines: though WT virus is highly dependent on these factors, CA mutant viruses such as N74D can bypass CPSF6 binding and infect cells depleted for NUP358, NUP153, or TNPO3 without a concomitant loss of infectivity. The gene discussed is CPSF6; the disease is HIV-1 infection.