In addition, Hes1 level was increased in H9c2 cells treated by LV-N1ICD infection and H/R, but decreased in H9c2 cells treated by LV-N1ICD-shRNA infection and IPC or IPost (Figure 2B), confirming that Hes1 is a target gene of Notch signaling. The gene discussed is HES1; the disease is infection.