Notably, the difference in the amastigotes numbers of the macrophages derived from the knockouts and the WT mice was not related to the invasion rate of T. cruzi, as similar numbers of amastigotes were observed in the macrophages from WT, NLRP3−/− and caspase-1−/− mice after 2 h of infection (Figure S1A). Here, CASP1 is linked to infection.